Stem Cell Signature Treatments – SEFFILLER Technique

The body’s own stem cells can replace damaged cells, rejuvenating the skin or restoring hair growth, for example. The possibilities are endless.

(Picture: SEFFILLER/SEFFIHAIR training at UMA clinic te Amsterdam)

Autologous regenerative therapy with the body's own stem cells. SEFFILLER training at UMA clinic in Amsterdam.

Stimulate hair growth with SEFFIHAIR®

Stop hair loss and stimulate new hair growth with the body’s own stem cells.

Stem cells from fat for natural skin rejuvenation – a safe alternative to fillers

A naturally rejuvenating result that can last for years.

Natural Skin Rejuvenation with Your Own Stem Cells

At Dr. Brandt Clinic in Amsterdam, our Stem Cell Signature Treatments, performed using the advanced SEFFILLER technique, utilize your own stem cell-rich fat for natural and safe rejuvenation of the skin and underlying tissue. These treatments combine the power of your body with the latest insights from stem cell therapy, PRP (Platelet-Rich Plasma), and regenerative medicine.

This approach allows the skin to be naturally renewed, firmed, and revitalized, providing a sustainable, body-based alternative to traditional injectables. Treatments are suitable for the face, neck, hands, décolleté, body areas such as hip dips, and intimate zones, and are fully tailored to your individual goals and needs.

Our Stem Cell Signature Treatments combine your own fat-derived stem cells with several scientifically validated techniques for an optimal and natural result:

Microneedling: stimulates natural collagen and elastin production for smoother, more even skin.
RF Microneedling / Morpheus8: firms and lifts the skin using controlled heat energy deep in the dermal layers.
Endolifting: a laser treatment that stimulates collagen remodeling from within for a fresher, tighter appearance.
Microfat- and Nanofat Therapy / Lipofilling: nourishes and regenerates the skin using your own stem cell-rich fat, as a natural alternative to fillers.
Optional PRP: can be combined to accelerate healing and further stimulate collagen production.

By combining these techniques, a powerful regenerative effect is achieved – a natural, sustainable, and body-based alternative to traditional injectables and fillers.

Looking for a natural skin-firming treatment, PRP or stem cell therapy, or a non-surgical anti-aging solution in Amsterdam? Our Stem Cell Signature Treatments, performed with the SEFFILLER technique at Dr. Brandt Clinic in Amsterdam, offer a scientifically based and holistic approach for a youthful, healthy-looking complexion.

Book your consultation

Curious to learn what natural treatments with stem cells and PRP can do for you? At Kliniek Dokter Brandt, we take the time to understand your needs and expectations. During a personal consultation, our experienced doctors will provide tailored advice, ensuring a natural and long-lasting result.

Before and after regenerative facial treatment using stem cells, Seffiller, lipofilling, micro- and nanofat in Amsterdam

Before and after regenerative facial treatment using stem cells, Seffiller, lipofilling, micro- and nanofat combined with Morpheus8 microneedling and radiofrequency in Amsterdam

Stem cells regenerative facial before after amsterdam 4

Before and after regenerative facial treatment using stem cells, Seffiller, lipofilling, micro- and nanofat combined with Radiesse collagen-stimulating filler in Amsterdam

Before and after regenerative facial treatment using stem cells for natural volume restoration and skin rejuvenation in Amsterdam

"We see a trend where people are increasingly seeking natural alternatives for a fresher appearance." Christopher J. Brandt - aesthetic physician KNMG

SEFFILLER® – Natural rejuvenation with your own fat & stem cells

SEFFILLER® is a regenerative treatment that uses your own fat tissue to naturally rejuvenate the skin. By utilizing superficial fat – rich in stem cells and growth factors – the skin is stimulated to repair and renew itself. The result is firmer, fuller skin and a youthful, refreshed appearance, without the use of synthetic fillers.

As we age, the production of collagen and elastin decreases. This leads to a loss of volume and elasticity, causing fine lines, wrinkles and sagging. SEFFILLER® addresses these changes at their source by harnessing the body’s own regenerative capacity. During the treatment, a small amount of fat is carefully harvested, processed, and then injected into areas that benefit from extra volume and firmness, such as the face, neck, décolleté, or hands. The fat cells provide immediate volume, while the stem cells stimulate the long-term production of collagen and elastin.

The effectiveness of SEFFILLER® is supported by extensive scientific research. Numerous studies have demonstrated how stem cells and growth factors found in fat tissue contribute to skin improvement and regeneration, making SEFFILLER® a safe and reliable choice for natural-looking results.

SEFFILLER® can also be perfectly combined with other treatments, such as fillers, botulinum toxin, microneedling, or chemical peels, to further enhance and refine the outcome.

Microfat and nanofat: the difference

Within fat grafting techniques, a distinction is made between microfat and nanofat. Microfat contains intact fat cells and is primarily used to restore volume. It is ideal for treating hollow areas such as the cheeks, temples, or lips, and for softening facial contours. The results are immediately visible and long-lasting.

Nanofat, on the other hand, is processed into a much finer, liquid form. This leaves almost no fat cells intact, but creates a solution rich in stem cells and growth factors. Nanofat is injected superficially into the skin, where it improves overall skin quality. It does not add volume, but refines the skin texture, reduces fine lines, and results in firmer, smoother, and more radiant skin.

In short, microfat is mainly used for volume restoration, while nanofat focuses on skin rejuvenation. In many cases, both methods are combined: microfat for contour and structure, nanofat for healthy, youthful-looking skin.

Unique combination treatments with stem cells

Stem cells with microneedling

Using the SEFFILLER technique, we use a small amount of your own fat for a natural anti-aging treatment. The fat is purified and partly re-injected into the face for subtle volume restoration, while the remaining stem cells are delivered into the skin through microneedling to visibly improve skin quality. Microneedling and RF microneedling stimulate collagen, elastin, and blood circulation, creating—together with the stem cells—the ideal environment for regeneration. Research has also shown that stem cells have better survival and integration when RF microneedling is applied beforehand. This synergy enhances the results, producing a natural, long-lasting outcome with smoother, firmer, and more youthful-looking skin.

Stem cells with Endolift

Combining the skin-tightening effect of Endolift (stimulation with radiofrequency) with the regenerative capability of stem cells. This allows us to target specific areas such as laxity of the lower eyelids with focused stimulation from Endolift and afterwards boosting the results with the regenerative effects of stem cells. The Endolift treatment can also be applied to facial support points to restore youthful contours in the face. If there is significant skin laxity, an operative ‘facelift’ might be a more suitable starting option. Afterward, you can maintain and optimize the skin condition with treatments based on the body’s natural regenerative capacity. Discuss with your doctor which option is most suitable for you.

Stem cells with biostimulating injectables

Biostimulators like Radiesse, polynucleotides (Philart) and NCTF have a stimulating effect on the cells in our skin that create volume, support and healthy blood supply. By combining these into one treatment we see synergistic effects, boosting the results even further. Weather we are looking to restore volume, create youthful contours in the face, treat skin laxity or restore hairgrowth; these combination treatments are amoung the most effective and natural options on the market today.

What does a treatment look like?

Step 1: Harvesting the stem cells

In the superficial fat, a significant number of stem cells are present. We can easily extract a small amount of this superficial fat tissue using a syringe under local anesthesia. Typically, the abdomen, flanks, or legs are commonly chosen for this procedure. The amount we remove is approximately equivalent to a shot of espresso (±30 ml.), so it’s not comparable to a typical liposuction. The area where the fat is ‘harvested’ may feel slightly tender for a few days after the procedure.

Step 2: Filtering the stem cells

After harvesting the fat, the next step involves filtering the stem cells. This can be done in various ways depending on the desired effect. If we aim for both skin improvement and a volumizing effect in the face, we will reintroduce the fat cells and filter out the anesthetic solution. For treatments focused on restoring hair growth, we also filter out the fat cells using a centrifuge. This leaves behind a fluid rich in growth factors and stem cells.

Step 3: The re-implantation of the stem cells

Depending on the desired effect, the stem cells may or may not be re-implanted in combination with the fat cells. In a SEFFILLER treatment, we reintroduce the mix of stem cells and fat cells into the subcutaneous fat of the face using a small cannula. In a SEFFIHAIR treatment, we place the filtered stem cells (without fat cells) back into the scalp using a ‘multi-injector’ that allows us to administer multiple small injections simultaneously. The treatment is performed under local anesthesia using a numbing injection or cream.

Step 4: Recovery and results

In the first few days after the treatment, the skin needs some time to settle. Expect mild swelling, redness, and a slight tenderness. It’s possible to encounter a small blood vessel during the procedure, resulting in a bruise, especially in the area where the stem cells were harvested. Generally, the recovery is mild, and beautiful results are often visible quickly. Optimal results will become apparent once the stem cells have settled in, multiplied, and started replacing old damaged cells. This typically occurs within 3-6 months. Depending on your situation and the desired effect, the treatment may be performed in a course of 2 or 3 sessions for optimal results. The results can last for years.

SEFFILLER Amsterdam

SEFFILLER is a groundbreaking ‘fat transfer’ procedure that provides a younger and fuller appearance by harnessing the body’s natural regenerative ability of stem cells. The superficial subcutaneous fat contains significant quantities of ‘mesenchymal stem cells’ (ADSCs) and ‘stromal vascular fraction’ (SVF), in simpler terms: stem cells and growth factors that prompt your skin to regenerate cells, restore volume, stimulate collagen, elastin, new blood vessels, and more. The result: skin improvement and facial rejuvenation. In contrast to temporary fillers, the results can last for years.

SEFFIHAIR Amsterdam

Stop hair loss and stimulate new hair growth met SEFFIHAIR

Hair loss affects 70% of all men and 40% of all women, often having a significant impact on self-image. In men, the overproduction of the enzyme dihydrotestosterone (DHT) plays a crucial role in weakening hair follicles, leading to the typical male pattern baldness with a receding hairline and hair loss on the top of the head. In women, hormonal changes can contribute to hair loss, typically leaving the hairline intact but causing visible thinning of the hair.

Frequently asked questions

What is SEFFILLER?

SEFFILLER is a groundbreaking ‘fat transfer’ procedure that provides a younger and fuller appearance by harnessing the body’s natural regenerative ability of stem cells. The superficial subcutaneous fat contains significant quantities of ‘mesenchymal stem cells’ (ADSCs) and ‘stromal vascular fraction’ (SVF), in simpler terms: stem cells and growth factors that prompt your skin to regenerate cells, restore volume, stimulate collagen, elastin, new blood vessels, and more. The result: skin improvement and facial rejuvenation. Unlike temporary fillers, the results can last for years.

What is SEFFIHAIR?

Hair loss affects 70% of all men and 40% of all women and can significantly impact self-esteem. In men, overproduction of the enzyme dihydrotestosterone (DHT) plays a crucial role in weakening hair follicles, leading to the typical male pattern baldness with a receding hairline (‘temples’) and hair loss on the top of the head. In women, hormonal changes can contribute to hair loss, typically leaving the hairline intact but causing visible thinning of the hair.

SEFFIHAIR is a medical treatment based on ‘autologous regenerative therapy’ (ART), where stem cells are extracted from the body’s own fat and re-implanted into the hairy scalp to stimulate new hair growth. This new technology has been proven safe, effective, and entirely natural.

What is the downtime?

The downtime is minimal and is limited to some local skin reactions at the injection sites (mild redness, swelling, and possibly a bruise for 24-72 hours). The area where the fat is ‘harvested’ may feel slightly tender for a few days. You can comfortably use makeup to conceal any post-treatment effects.

When do I see results?

After a SEFFILLER treatment for the face, you’ll immediately notice a difference due to the volumizing effect of the transplanted fat cells. Around 8-12 weeks later, you’ll observe visible skin improvement; your skin becomes firmer and tighter. The complexion and skin hydration also improve. Optimal results are seen around 6 months, and these results can last for years.

Is a treatment with stem cells safe?

In recent years, extensive scientific research has been conducted on treatments using the body’s own stem cells (ADSCs and SVF), demonstrating not only their high effectiveness but also the minimal risk of side effects. It’s an ‘autologous’ treatment, meaning your own tissue is used. In other words, nothing foreign enters your body. The treatment should be performed by a certified medical professional such as a ‘cosmetic physician KNMG,’ dermatologist, or plastic surgeon with proven experience in administering fillers or autologous fat ‘lipofilling.’

How many treatments are necessary?

Depending on the condition of the skin or the extent of hair loss, it may be beneficial to repeat the treatment. Repeating the procedure can provide an additional ‘boost’ to the regenerative process, enhancing the visibility of even more beautiful results. It is recommended to repeat the treatment after 3 months. Discuss with your doctor how many sessions are necessary in your case to achieve the desired effect.